10x PBS Buffer 1 L
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10x PBS Buffer 1 L
Each 10X PBS solution is ready to use upon dilution to the desired concentration.
10x PBS Buffer 1 L
Numéro d'article: 90238222

10x PBS Buffer 1 L

Numéro d'article: 90238222

XOF 36269

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Each 10X PBS solution is ready to use upon dilution to the desired concentration.
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Détails du produit

Shop 10x PBS Buffer 1 L online at a best price in Benin. B0DJCP1GGX
Item Weight2.2 lbs (1 kg)

DESCRIPTION DU PRODUIT

10x PBS Buffer 1 L

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Questions et réponses des clients

  • question: What is 10x PBS Buffer used for?

    répondre: 10x PBS Buffer is primarily used in biological and biochemical research as a buffering agent. It helps maintain a stable pH level when diluting samples, making it ideal for cell culture, the dilution of proteins, and washing of cells. Its composition of sodium phosphate, sodium chloride, and potassium chloride provides ionic strength, which is crucial for assays, such as Western blots and ELISA. For example, when preparing cell cultures, using PBS ensures that the cells remain healthy and viable for experimentation.
  • question: How do you prepare a working solution from 10x PBS Buffer?

    répondre: To prepare a working solution from 10x PBS Buffer, simply dilute it with distilled water. For most applications, a common dilution used is 1 part 10x PBS to 9 parts water, resulting in a 1x PBS solution. Make sure to mix thoroughly. This dilution is ready for use in applications such as washing cells before experiments or as a diluent for biological samples. This preparation process is straightforward, ensuring that you can have a consistent buffer for various experimental procedures.
  • question: Is 10x PBS Buffer suitable for mammalian cell culture?

    répondre: Yes, 10x PBS Buffer is highly suitable for mammalian cell culture. It provides the necessary ionic environment to stabilize the cells during various biological processes. In cell culture, PBS is often used for rinsing the cells, removing media, or resuspending them without causing damage. As a result, it ensures that cells remain in optimal condition for experiments and assays, enhancing the reliability of your results.
  • question: What is the difference between 1x PBS and 10x PBS?

    répondre: The main difference between 1x PBS and 10x PBS lies in their concentration. 10x PBS is ten times more concentrated than the 1x formulation. The 10x PBS buffer is typically diluted before use to achieve the desired concentration of 1x, which is the standard working concentration for many laboratory applications. For instance, if you're conducting an experiment that requires a washing step, you’ll use the diluted 1x PBS to ensure optimal pH and ionic strength without affecting your results.
  • question: Can 10x PBS Buffer be used for protein storage?

    répondre: While 10x PBS Buffer is excellent for preparing solutions, it is not ideal for long-term protein storage. Proteins are generally more stable in solutions that contain stabilizers such as glycerol or specific buffer conditions tailored for the protein's stability. Instead, 10x PBS can be used to dilute proteins for immediate use in assays or experiments. Always consider the nature of your protein and consult relevant literature to choose the best storage conditions.
  • question: How should 10x PBS Buffer be stored?

    répondre: 10x PBS Buffer should be stored at room temperature or in a refrigerator, depending on the specific formulation and any added components. Avoid exposure to extreme conditions that might alter its pH or ionic concentrations. Once opened, it’s essential to keep it tightly sealed to prevent contamination. Proper storage ensures that the buffer remains effective for various laboratory applications, thus maintaining the integrity of your experimental results.
  • question: Is 10x PBS Buffer toxic?

    répondre: 10x PBS Buffer is generally considered non-toxic and safe for handling in laboratory environments. However, standard laboratory safety protocols should always be followed. Use gloves and goggles when handling any chemical solutions to protect yourself from potential irritants. Despite its safety profile, awareness of proper disposal methods and material safety data sheets (MSDS) for the components used in PBS can ensure safe handling practices.
  • question: What are the typical applications of 10x PBS Buffer in research?

    répondre: 10x PBS Buffer is utilized in a wide range of research applications, including cell washing, sample dilution, and protein assays. It is commonly used in immunological techniques, such as ELISA and Western blots, where maintaining a stable pH and ionic strength is crucial. Additionally, it's indispensable in the preparation and dilution of biological samples for microscopy and flow cytometry. This versatility makes 10x PBS a fundamental tool in many research laboratories.
  • question: Can I use 10x PBS Buffer with live cell imaging?

    répondre: Yes, 10x PBS Buffer can be used for live cell imaging; however, it must first be diluted to a 1x concentration to avoid hyperosmolarity that could affect cell health. In live cell imaging experiments, 1x PBS can serve as a wash buffer before adding imaging media. Its balanced ionic strength helps maintain cell integrity during microscopy, making it suitable for various imaging techniques, including fluorescence and confocal microscopy.
  • question: Where can I buy 10x PBS Buffer (1 L) in Benin?

    répondre: You can buy 10x PBS Buffer (1 L) from Ubuy in Benin. Ubuy offers a wide selection of laboratory reagents and supplies, making it easy to find quality products like 10x PBS. So, if you're looking to purchase this essential buffer, Ubuy is your go-to source for reliable lab supplies.

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Avantages

  • High quality reagents
  • Stable and long shelf life
  • Versatile buffer application
  • Convenient 1 L size
  • Accurate pH maintenance

Les inconvénients

  • Occasional precipitation issue

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Informations importantes

  • Limitations : Pour les produits expédiés à l'international, veuillez noter que toute garantie du fabricant peut ne pas être valide ; les options de service du fabricant peuvent ne pas être disponibles ; les manuels, instructions et avertissements de sécurité des produits peuvent ne pas être dans les langues du pays de destination ; les produits (et les matériaux qui les accompagnent) peuvent ne pas être conçus conformément aux normes, spécifications et exigences d'étiquetage du pays de destination ; et les produits peuvent ne pas être conformes à la tension et aux autres normes électriques du pays de destination (nécessitant l'utilisation d'un adaptateur ou d'un convertisseur le cas échéant). Il incombe au destinataire de s'assurer que le produit peut être importé légalement dans le pays de destination. En cas de commande auprès d'Ubuy ou de ses filiales, le destinataire est l'importateur officiel et doit se conformer à toutes les lois et réglementations du pays de destination.
  • Tous les produits listés sur Ubuy ne sont pas à vendre, Ubuy étant un moteur de recherche mondial. Les produits sont soumis aux réglementations en matière d'exportation et de commerce.

XOF 36269

Commandez maintenant et recevez votre commande aux alentours du Monday, Juillet 13

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Ubuy s'engage à protéger votre sécurité et votre confidentialité. Notre système avancé de sécurité des paiements garantit la confidentialité en chiffrant vos informations lors de la transmission grâce aux protocoles AES (Advanced Encryption Standards) et SSL (Secure Socket Layer). Vos coordonnées de paiement sont 100 % sécurisées car nous ne partageons pas vos informations de paiement avec des vendeurs tiers.

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Caractéristiques et avantages

  • Ready-to-use 10X PBS solution for your laboratory needs.
  • Convenient dilution to 1X working concentration for quick application.
  • Ideal for Western blot, ELISA, and immunoprecipitation.
  • Formulated with ultrapure-grade phosphate buffers and saline solutions.
  • Clear colorless liquid with a pH of 7.4 ± 0.2.
  • Filtered with a 0.45µm filter ensuring high purity.