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Item #:
160749865
10mM Ammonium Bicarbonate Buffer, pH 8.0, 250mL
Item #:
160749865
XOF 71234
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Manufactured in an ISO 9001 and 13485 certified facility in the USA, our buffers are vital for protein research and molecular biology.
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What Stands Out
Precise pH Control
Maintains a stable pH of 8.0, crucial for biochemical analyses, providing reliable results in experiments while minimizing variation in outcomes.
Ammonium Bicarbonate Solution
Formulated as a 10mM solution, it serves as a versatile buffer for proteins and nucleic acids, enhancing separation and stabilization during various laboratory procedures.
Convenient Volume
Offered in a 250mL bottle, it ensures ample supply for repeated experiments, reducing the need for frequent re-preparation and saving time in busy lab settings.
Product Details
| Container Type | Bottle |
| Item Form | Gel |
| Item Weight | 4 ounces (113.4 grams) |
| Volume | 250 ml |
| Brand Name | eCon Lab Supply |
| Manufacturer | Ciro Mfg |
| Included Components | Bottle |
| Part Number | 23.105.102 |
| Brand | eCon Lab Supply |
| Item Volume | 250 ml |
| Package Information | Bottle |
Who Should Buy?
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Biochemists
Ideal for biochemists needing a stable buffer for enzymatic reactions and protein stabilization at pH 8.0.
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Chromatography Labs
Perfect for labs engaged in chromatography where a consistent buffer is essential for optimal separation.
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Research Institutions
Essential for research settings requiring reliable biological buffers for various experimental protocols and methodologies.
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Acidic Solutions
Not suitable for applications requiring acidic conditions, as the pH is set to 8.0.
Product Description
10mM Ammonium Bicarbonate Buffer, pH 8.0, 250mL
Have any Query? Chat with us
Customer Questions & Answers
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Question:
What is 10mM Ammonium Bicarbonate Buffer used for?
Answer: 10mM Ammonium Bicarbonate Buffer is commonly used in biochemical and molecular biology applications, particularly for protein purification and stabilization. This buffer maintains a stable pH of 8.0, making it ideal for enzyme assays and chromatography. It effectively stabilizes proteins and nucleic acids in various experimental settings, ensuring optimal activity and structural integrity. -
Question:
How should I store 10mM Ammonium Bicarbonate Buffer?
Answer: It is recommended to store 10mM Ammonium Bicarbonate Buffer at room temperature, away from direct sunlight. For long-term storage, refrigeration is advisable to prevent degradation. Always ensure the container is tightly sealed to avoid contamination and evaporation, which can affect the buffer's pH and efficacy. -
Question:
Can I use 10mM Ammonium Bicarbonate Buffer for cell culture?
Answer: While 10mM Ammonium Bicarbonate Buffer is primarily used in biochemical analyses, it is not ideal for standard cell culture since it can alter the pH of the culture medium. It’s better suited for enzymatic reactions or for use in conjunction with other buffers tailored specifically for mammalian cultures, ensuring optimal growth conditions. -
Question:
What pH range can I expect from 10mM Ammonium Bicarbonate Buffer?
Answer: As indicated, the pH of 10mM Ammonium Bicarbonate Buffer is stabilized at 8.0. This pH is crucial for maintaining the activity of many biochemical reactions. It plays a significant role in protein interaction studies, where maintaining an appropriate pH ensures accurate and reproducible results. -
Question:
Is 10mM Ammonium Bicarbonate Buffer toxic?
Answer: 10mM Ammonium Bicarbonate Buffer is generally considered nontoxic and safe to handle. However, standard laboratory safety practices should always be followed, including the use of gloves and goggles when handling chemicals, to avoid any potential irritation or accidental ingestion. -
Question:
What are the benefits of using a buffer with pH 8.0?
Answer: A buffer with pH 8.0, such as 10mM Ammonium Bicarbonate, is advantageous for reactions involving proteins and nucleic acids. At this pH, many enzymes maintain optimal activity, and it stabilizes molecules that require a slightly alkaline environment. This buffer is crucial in experiments focusing on enzymatic reactions or studies involving biomolecules. -
Question:
Can I prepare my own 10mM Ammonium Bicarbonate Buffer?
Answer: Yes, preparing your own 10mM Ammonium Bicarbonate Buffer involves dissolving the appropriate amount of ammonium bicarbonate in distilled water to achieve the desired molarity. It’s important to use a pH meter to confirm the buffer maintains a pH of 8.0, as accurate buffering is essential for reliable experimental outcomes. -
Question:
What industries commonly use 10mM Ammonium Bicarbonate Buffer?
Answer: 10mM Ammonium Bicarbonate Buffer is frequently utilized in research laboratories across various industries, including pharmaceuticals, biotechnology, and academia. It finds application in procedures related to protein analysis, gene studies, and therapeutic development, making it an integral part of many research protocols. -
Question:
What sizes does 10mM Ammonium Bicarbonate Buffer come in?
Answer: 10mM Ammonium Bicarbonate Buffer is available in various volumes, with 250mL being a commonly offered size. This allows researchers to choose based on their experimental needs while ensuring sufficient buffer for multiple assays or tests without frequent preparation. -
Question:
Where can I buy 10mM Ammonium Bicarbonate Buffer, PH 8.0, 250mL?
Answer: You can buy 10mM Ammonium Bicarbonate Buffer, PH 8.0, 250mL at Ubuy. They offer a wide selection of lab chemicals and supplies, ensuring you have access to high-quality products for your research needs.
eCon Lab Supply Buffers Editorial Review
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Features & Benefits
- Comprehensive range of biological buffers available.
- Ideal for research, diagnostics, drug development, and manufacturing.
- Produced in a certified ISO 9001 and 13485 facility.
- Subject to strict Good Manufacturing Practices and quality control.
- Effective for protein digestion and deglycosylation reactions.
- Suitable for 2D and 3D electrophoretic gel applications.
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